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The Peptide Effect

Tools

Injection Volume Calculator

Enter your desired dose and peptide concentration to calculate the exact volume in mL and units to draw on a U-100 insulin syringe.

Quick presets

mcg
mg/mL

Syringe rounding

Draw on syringe (U-100)

5.00

units (IU)

Volume

0.0500

mL

Exact units

5.00

IU (unrounded)

Show the math

Convert dose: 250 mcg ÷ 1000 = 0.25 mg

Volume: 0.25 mg ÷ 5 mg/mL = 0.0500 mL

Syringe units: 0.0500 mL × 100 IU/mL = 5.00 IU

Assumptions

  • Assumes a standard U-100 insulin syringe (1 mL = 100 units)
  • Concentration depends on your reconstitution — verify before drawing
  • Rounding introduces small dosage error; “Exact” shows raw calculation
  • Always double-check your math before administering any injection

How This Calculator Works

Flow diagram showing how the injection volume calculator works
Volume calculation flow — from dose to syringe units

The calculator converts your desired dose into a volume using the formula: volume (mL) = dose (mg) ÷ concentration (mg/mL). It then converts mL to insulin syringe units using the U-100 standard where 1 mL = 100 units.

If your dose is in micrograms (mcg), the calculator first converts to milligrams by dividing by 1,000. Optional rounding snaps the result to the nearest mark on your syringe.

Assumptions & Limitations

Frequently Asked Questions

How do I convert my dose to syringe units?
Divide your dose (in mg) by the concentration (in mg/mL) to get the volume in mL. Then multiply by 100 to convert to insulin units on a U-100 syringe. For example, 250 mcg (0.25 mg) at a 5 mg/mL concentration = 0.05 mL = 5 units.
What is a U-100 insulin syringe?
A U-100 syringe is calibrated so that 100 units = 1 mL. These are the standard insulin syringes used for subcutaneous peptide injections. Common sizes are 1 mL (100 unit), 0.5 mL (50 unit), and 0.3 mL (30 unit).
What concentration should I enter?
Your concentration depends on how you reconstituted the peptide. For example, if you added 2 mL of bacteriostatic water to a 10 mg vial, the concentration is 10 mg / 2 mL = 5 mg/mL. Use our reconstitution calculator if you need help determining your concentration.
Should I round the syringe units?
Most insulin syringes have markings at every 1 or 2 units. If your calculated volume falls between marks, rounding to the nearest unit is practical. The “Exact” mode shows unrounded values so you can see how much rounding changes the dose.

Educational Use Only

This calculator is provided for educational and informational purposes. It is not medical advice. Always verify your calculations independently and consult a healthcare provider before administering any injection.

Last reviewed: February 2026