Ghk Cu Reconstitution: Step-by-Step Mixing Instructions

Overview

Reconstituting GHK-Cu means dissolving the lyophilized (freeze-dried) powder into an injectable solution using bacteriostatic water. This guide walks through the process step by step, including how to calculate your concentration after mixing. GHK-Cu has Phase 2 human data, but evidence remains incomplete and may not generalize. This is educational information — always follow your prescriber's specific instructions.

What Reconstitution Means for GHK-Cu

Many peptides, including GHK-Cu, are supplied as a lyophilized (freeze-dried) powder. This powder must be dissolved in a sterile diluent — typically bacteriostatic water (BAC water) — before it can be injected. This process is called reconstitution. GHK-Cu has Phase 2 human data, but evidence remains incomplete and may not generalize. The reconstitution process does not change the peptide itself; it simply creates an injectable solution at a known concentration.

What You Need for Reconstitution

Before reconstituting, gather all supplies in a clean workspace. Contamination during reconstitution is a significant safety concern.

• Bacteriostatic water (BAC water) — contains 0.9% benzyl alcohol as a preservative
• Alcohol swabs for cleaning vial stoppers
• A syringe for drawing and transferring the diluent (typically 1-3 mL)
• The peptide vial (lyophilized powder)
• A clean, flat workspace away from contaminants

Step-by-Step Reconstitution Procedure

The following is a general educational guide. Always follow the specific reconstitution instructions from your prescriber or pharmacy label if they differ from these general steps.

• Wash hands thoroughly with soap and water
• Clean the stoppers of both the peptide vial and BAC water vial with alcohol swabs
• Draw the desired volume of BAC water into the syringe (the volume depends on your target concentration)
• Insert the needle into the peptide vial at an angle, aiming at the inside wall of the vial
• Inject the BAC water slowly, letting it run down the side of the vial — do NOT squirt directly onto the powder
• Remove the syringe gently
• Swirl the vial very gently in a circular motion — NEVER shake, as this can denature the peptide
• Wait for the powder to dissolve completely (may take 1-5 minutes); the solution should be clear

Calculating Your Dose After Reconstitution

After reconstitution, you need to know the concentration to draw the correct dose. The formula is simple: concentration = total peptide (in mcg or mg) divided by total water volume (in mL). For example, if you have a 5 mg vial and add 2 mL of BAC water, the concentration is 2.5 mg/mL (or 2,500 mcg/mL). Our reconstitution calculator tool can do this math for you.

• Concentration = total peptide amount ÷ total diluent volume
• Dose volume = desired dose ÷ concentration
• Example: 250 mcg dose from a 2,500 mcg/mL solution = 0.1 mL (10 units on a U-100 insulin syringe)
• Always double-check math before drawing — dosing errors are the most common reconstitution mistake

Storage After Reconstitution

Once reconstituted, GHK-Cu solution must be refrigerated at 2-8°C (36-46°F). Reconstituted peptides are less stable than the lyophilized powder form and will degrade over time even when properly stored. Most reconstituted peptide solutions maintain potency for approximately 14-28 days when refrigerated, though specific stability data varies by compound. Never freeze reconstituted peptide solutions.

• Refrigerate immediately after reconstitution (2-8°C / 36-46°F)
• Most reconstituted peptides remain usable for 14-28 days (compound-specific)
• Protect from light — store in the original vial
• If the solution becomes cloudy, discolored, or contains visible particles, discard it

Common Reconstitution Mistakes

These are the most frequently reported errors during peptide reconstitution. Avoiding them improves both safety and consistency.

• Shaking the vial vigorously — this can denature the peptide and reduce potency
• Squirting BAC water directly onto the powder — aim at the vial wall instead
• Using sterile water instead of bacteriostatic water — sterile water lacks preservative, limiting multi-use
• Adding the wrong volume of diluent — this changes your concentration and every subsequent dose
• Not cleaning vial stoppers with alcohol — contamination risk
• Storing reconstituted solution at room temperature — accelerates degradation

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References

1. GHK peptide as a natural modulator of multiple cellular pathways in skin regeneration (2015) — PubMed
2. GHK-Cu may prevent oxidative stress in skin by regulating copper and modifying expression of numerous antioxidant genes (2012) — PubMed
3. Stability of reconstituted lyophilized peptide formulations (2017) — PubMed
4. Good practice guide for the reconstitution, administration and storage of parenteral products (2016) — PubMed